Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/Entry Base"

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==CNHY Acos5==
+
==CNTI Acos5==
  
  
 
===PCR===
 
===PCR===
* [[Image:20111023_PCRa.png|800 px]]
+
* [[Image:20120110_PCRa.png|800 px]]
  
  
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[[Image:20111023_Gel.png|800 px]]
+
[[Image:20120110_Gela.png|800 px]]
  
 
* mispriming everywhere - primer pair not usable for CNHY
 
* mispriming everywhere - primer pair not usable for CNHY

Revision as of 09:01, 10 January 2012

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR

  • 20120110 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20120110 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png