User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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== | ==CNHY Acos5== | ||
=== | ===PCR=== | ||
* | * [[Image:20111023_PCRa.png|800 px]] | ||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20111023_Gel.png|800 px]] | |||
* mispriming everywhere - primer pair not usable for CNHY | |||
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | |||
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]] | |||
Revision as of 16:58, 29 October 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | |||||||||
CNHY Acos5PCR
Gel
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