Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/Entry Base"

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==Task==
+
==CNHY Acos5==
* here
 
* here
 
** in here
 
# numbered
 
# again
 
  
  
===Subsection===
+
===PCR===
* here
+
* [[Image:20111023_PCRa.png|800 px]]
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20111023_Gel.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for CNHY
 +
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 +
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 +
 
  
  

Revision as of 15:58, 29 October 2011

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNHY Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


800 px

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png