Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/06/06"

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(Autocreate 2013/06/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==SCOC MP2 Plates1-4==
  
  
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* comments
+
* Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)
:: [[Image:20130110_PCRa.png|800 px]]
+
:: [[Image:20130606_PCRa1.png|800 px]]
 +
:: [[Image:20130606_PCRa2.png|800 px]]
  
  
===Gel===
+
==ASHY MP1/2/3 Opt==
  
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
 
+
===PCR===
:: [[Image:20130110_Gela.png|800 px]]
 
  
  
* mispriming everywhere - primer pair not usable for CNHY
+
* Re-do from 18Mar2013 (everything failed unexpectedly)
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
:: [[Image:20130606_PCRb.png|800 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
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* UAGC Submission# ????
+
* UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF
:: [[Image:20130227_Frag.png|900 px]]
+
:: [[Image:20130606_Fraga.png|900 px]]
 +
:: [[Image:20130606_Fragb.png|900 px]]
  
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
+
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713100 801124713100]
:: [[Image:20130227_FedEx.png|700 px]]
+
:: [[Image:20130606_FedEx.png|700 px]]
  
  

Revision as of 13:49, 9 June 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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SCOC MP2 Plates1-4

PCR

  • Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)
20130606 PCRa1.png
20130606 PCRa2.png


ASHY MP1/2/3 Opt

PCR

  • Re-do from 18Mar2013 (everything failed unexpectedly)
20130606 PCRb.png


Fragment Analysis Submission

  • UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF
20130606 Fraga.png
20130606 Fragb.png


700 px


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February