Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/05/22"

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* SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
 
* SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
 
:: [[Image:20130522_PCR.png|800 px]]
 
:: [[Image:20130522_PCR.png|800 px]]
 +
* Complications:
 +
** I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.
  
  

Revision as of 08:08, 23 May 2013

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SCOC MP2

PCR

  • From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
  • SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
20130522 PCR.png
  • Complications:
    • I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.


Fragment Analysis Submission

20130522 Frag.png


20130522 FedEx.png


  • UAGC Submission# HBXH


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February