Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/05/15"

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(Autocreate 2013/05/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==SCOC MP2==
 +
Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.
  
  
 
===PCR===
 
===PCR===
* [[Image:20130110_PCRa.png|800 px]]
+
* [[Image:20130515_PCR.png|800 px]]
 
 
 
 
===Gel===
 
 
 
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
 
 
[[Image:20130110_Gela.png|800 px]]
 
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
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:: [[Image:20130227_Frag.png|900 px]]
+
:: [[Image:20130515_Frag.png|900 px]]
  
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
+
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713155 801124713155]
:: [[Image:20130227_FedEx.png|700 px]]
+
:: [[Image:20130515_FedEx.png|700 px]]
  
  
* UAGC Submission# ????
+
* UAGC Submission# PZZN
  
  
* FedEx picked up ____pm, 26 February
+
* FedEx picked up ____pm, 15 May
* FedEx delivered ____am, 27 February
+
* FedEx delivered ____am, 16 May
* UAGC received ____am, 27 February
+
* UAGC received ____am, 16 May
* UAGC completed ____am, 28 February
+
* UAGC completed ____am, 17 May
  
  

Revision as of 13:27, 15 May 2013

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SCOC MP2

Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.


PCR

  • 20130515 PCR.png


Fragment Analysis Submission

20130515 Frag.png


20130515 FedEx.png


  • UAGC Submission# PZZN


  • FedEx picked up ____pm, 15 May
  • FedEx delivered ____am, 16 May
  • UAGC received ____am, 16 May
  • UAGC completed ____am, 17 May