Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/05/07"

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(Autocreate 2013/05/07 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==SCOC MP2==
  
  
===PCR===
+
* Since SCOC MP2 Plates 1-5 were plated a LONG time ago (early March-ish?), only trying/PCRing one plate to make sure dry DNA is still viable. If results from this plate (Plate 5) are clean, then use rest of pre-plated DNA, too!
* [[Image:20120110_PCRa.png|800 px]]
 
  
  
===Gel===
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===PCR===
 
+
* [[Image:20130507_PCR.png|800 px]]
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
 
 
[[Image:20120110_Gela.png|800 px]]
 
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
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:: [[Image:20130226_Frag.png|900 px]]
+
:: [[Image:20130507_Frag.png|900 px]]
  
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
+
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713166 801124713166]
:: [[Image:20130226_FedEx.png|700 px]]
+
:: [[Image:20130507_FedEx.png|700 px]]
  
  
* UAGC Submission# PPTX
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* UAGC Submission# VQCJ
  
  
* FedEx picked up 2:44pm, 26 February
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* FedEx picked up pm, 7 May
* FedEx delivered 9:29am, 27 February
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* FedEx delivered am, 8 May
* UAGC received 10:58pm, 27 February
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* UAGC received am, 8 May
* UAGC completed 10:41am, 28 February
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* UAGC completed am, 9 May
  
  

Revision as of 13:11, 7 May 2013

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SCOC MP2

  • Since SCOC MP2 Plates 1-5 were plated a LONG time ago (early March-ish?), only trying/PCRing one plate to make sure dry DNA is still viable. If results from this plate (Plate 5) are clean, then use rest of pre-plated DNA, too!


PCR

  • 20130507 PCR.png


Fragment Analysis Submission

20130507 Frag.png


20130507 FedEx.png


  • UAGC Submission# VQCJ


  • FedEx picked up pm, 7 May
  • FedEx delivered am, 8 May
  • UAGC received am, 8 May
  • UAGC completed am, 9 May