Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/04/22"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/04/22 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
(No difference)

Revision as of 13:43, 22 April 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


CNTI Acos5

PCR

  • 20120110 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20120110 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png


Fragment Analysis Submission

20130226 Frag.png


20130226 FedEx.png


  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February