Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/03/12"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/03/12 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
(One intermediate revision by the same user not shown)
Line 9: Line 9:
  
  
==CNTI Acos5==
+
==SCOC MP2 optimization==
 
 
 
 
===PCR===
 
* [[Image:20120110_PCRa.png|800 px]]
 
 
 
 
 
===Gel===
 
 
 
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
 
 
[[Image:20120110_Gela.png|800 px]]
 
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
Line 38: Line 15:
  
  
:: [[Image:20130226_Frag.png|900 px]]
+
:: [[Image:20130312_Frag.png|900 px]]
  
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
+
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151723 801124151723]
:: [[Image:20130226_FedEx.png|700 px]]
+
:: [[Image:20130312_FedEx.png|700 px]]
  
  
* UAGC Submission# PPTX
+
* UAGC Submission# DZAA
  
  
* FedEx picked up 2:44pm, 26 February
+
* FedEx picked up 2:57pm, 12 March
* FedEx delivered 9:29am, 27 February
+
* FedEx delivered ????am, 13 March
* UAGC received 10:58pm, 27 February
+
* UAGC received ????am, 13 March
* UAGC completed 10:41am, 28 February
+
* UAGC completed ????am, 14 March
  
  

Revision as of 16:26, 12 March 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP2 optimization

Fragment Analysis Submission

20130312 Frag.png


20130312 FedEx.png


  • UAGC Submission# DZAA


  • FedEx picked up 2:57pm, 12 March
  • FedEx delivered ????am, 13 March
  • UAGC received ????am, 13 March
  • UAGC completed ????am, 14 March