Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/03/11"

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(Autocreate 2013/03/11 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==ASHY MP1/2/3 optimization==
  
  
===PCR===
+
===Gel===
* [[Image:20120110_PCRa.png|800 px]]
+
 
 +
 
 +
[[Image:20130311_Gel.png|800 px]]
  
 +
* good enough - send
  
===Gel===
 
  
{| {{table}} style="text-align: center"
+
===Fragment Analysis Submission===
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
  
[[Image:20120110_Gela.png|800 px]]
+
:: [[Image:20130311_Frag.png|900 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
 +
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151734 801124151734]
 +
:: [[Image:20130311_FedEx.png|700 px]]
  
===Fragment Analysis Submission===
 
  
 +
* UAGC Submission# GZNG
  
:: [[Image:20130226_Frag.png|900 px]]
 
  
 +
* FedEx picked up 3:03pm, 11 March
 +
* FedEx delivered 9:09am, 12 March
 +
* UAGC received 10:59am, 12 March
 +
* UAGC completed ?????am, 13 March
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
 
:: [[Image:20130226_FedEx.png|700 px]]
 
  
 +
==ASHY MP1/2/3 optimization==
  
* UAGC Submission# PPTX
 
  
 +
===PCR===
 +
* [[Image:20120110_PCRa.png|800 px]]
  
* FedEx picked up 2:44pm, 26 February
 
* FedEx delivered 9:29am, 27 February
 
* UAGC received 10:58pm, 27 February
 
* UAGC completed 10:41am, 28 February
 
  
  

Revision as of 15:21, 12 March 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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ASHY MP1/2/3 optimization

Gel

20130311 Gel.png

  • good enough - send


Fragment Analysis Submission

20130311 Frag.png


20130311 FedEx.png


  • UAGC Submission# GZNG


  • FedEx picked up 3:03pm, 11 March
  • FedEx delivered 9:09am, 12 March
  • UAGC received 10:59am, 12 March
  • UAGC completed ?????am, 13 March


ASHY MP1/2/3 optimization

PCR

  • 20120110 PCRa.png