User:Tara K. Luckau/Notebook/Team ConGen/2013/03/09: Difference between revisions

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==CNTI Acos5==
==ASHY MP1/2/3 optimization==




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130309_PCRa.png|800 px]]




===Gel===
* [[Image:20130309_PCRb.png|800 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* [[Image:20130309_PCRc.png|800 px]]


[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
===Fragment Analysis Submission===
:: [[Image:20130226_Frag.png|900 px]]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
:: [[Image:20130226_FedEx.png|700 px]]
* UAGC Submission# PPTX
* FedEx picked up 2:44pm, 26 February
* FedEx delivered 9:29am, 27 February
* UAGC received 10:58pm, 27 February
* UAGC completed 10:41am, 28 February


* [[Image:20130309_PCRd.png|800 px]]




Revision as of 16:17, 12 March 2013

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ASHY MP1/2/3 optimization

PCR






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