Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/03/07"

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(Autocreate 2013/03/07 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==SCOC MP2 optimization==
  
  
 
===PCR===
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
+
* [[Image:20130307_PCR.png|800 px]]
  
  
 
===Gel===
 
===Gel===
  
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
 +
*for SCOC MP1 Batch4 and SCOC MP2 optimization
  
[[Image:20120110_Gela.png|800 px]]
 
  
* mispriming everywhere - primer pair not usable for CNHY
+
[[Image:20130307_Gel.png|800 px]]
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
* good enough - send
  
  
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:: [[Image:20130226_Frag.png|900 px]]
+
:: [[Image:20130307_Fraga.png|900 px]]
 +
:: [[Image:20130307_Fragb.png|900 px]]
  
  
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
+
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151745 801124151745]
:: [[Image:20130226_FedEx.png|700 px]]
+
:: [[Image:20130307_FedEx.png|700 px]]
  
  
* UAGC Submission# PPTX
+
* UAGC Submission# KWWX and YPFS
  
  

Revision as of 16:11, 12 March 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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SCOC MP2 optimization

PCR

  • 20130307 PCR.png


Gel

  • for SCOC MP1 Batch4 and SCOC MP2 optimization


20130307 Gel.png

  • good enough - send


Fragment Analysis Submission

20130307 Fraga.png
20130307 Fragb.png


20130307 FedEx.png


  • UAGC Submission# KWWX and YPFS


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February