Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26"

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(Autocreate 2013/02/26 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
 
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #9DB68C" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==CNTI Acos5==
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==SCOC MP2 Optimization==
  
  
===PCR===
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* uniplex SW markers to see quality of peaks and range of alleles
* [[Image:20120110_PCRa.png|800 px]]
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* multiplex SW markers as first glance of how multiplex behaves
  
  
 
===Gel===
 
===Gel===
  
{| {{table}} style="text-align: center"
 
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! Pour !! Load !! Run
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
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[[Image:20130226_Gel.png|800 px]]
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* gel floated sideways, so it looks funny, but whatevs
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* clean bands in uniplexes
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* multiplex has amplification
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* send to frag!
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===Fragment Analysis Submission===
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:: [[Image:20130226_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
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:: [[Image:20130226_FedEx.png|700 px]]
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* UAGC Submission# PPTX
  
[[Image:20120110_Gela.png|800 px]]
 
  
* mispriming everywhere - primer pair not usable for CNHY
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* FedEx picked up 2:44pm, 26 February
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* FedEx delivered 9:29am, 27 February
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* UAGC received 10:58pm, 27 February
 +
* UAGC completed 10:41am, 28 February
  
  

Latest revision as of 21:30, 26 September 2017

Owwnotebook icon.png Tara's Lab Notebook Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Gel

20130226 Gel.png


  • gel floated sideways, so it looks funny, but whatevs
  • clean bands in uniplexes
  • multiplex has amplification
  • send to frag!


Fragment Analysis Submission

20130226 Frag.png


20130226 FedEx.png


  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February