Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/02/25"

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(Autocreate 2013/02/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
(fix raw html notebook nav)
 
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
 
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #9DB68C" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==CNTI Acos5==
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==SCOC MP1 Batch 2 Plate 1&2 (re-do)==
  
  
===PCR===
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===Fragment Analysis Submission===
* [[Image:20120110_PCRa.png|800 px]]
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 +
 
 +
* combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)
 +
 
 +
 
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:: [[Image:20130225_Frag.png|900 px]]
 +
 
 +
 
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151675 801124151675]
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:: [[Image:20130225_FedEx.png|700 px]]
 +
 
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* UAGC Submission# XPTR & VMZX
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 +
 
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* FedEx picked up 2:41pm, 25 February
 +
* FedEx delivered 9:12am, 26 February
 +
* UAGC received 10:43am, 26 February
 +
* UAGC completed 9:28am, 27 February
 +
 
 +
 
 +
==SCOC MP2 Optimization==
 +
 
  
 +
* uniplex SW markers to see quality of peaks and range of alleles
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* multiplex SW markers as first glance of how multiplex behaves
  
===Gel===
 
  
{| {{table}} style="text-align: center"
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===PCR===
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
  
[[Image:20120110_Gela.png|800 px]]
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* [[Image:20130225_PCRa.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
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* [[Image:20130225_PCRb.png|800 px]]
  
  

Latest revision as of 21:30, 26 September 2017

Owwnotebook icon.png Tara's Lab Notebook Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


SCOC MP1 Batch 2 Plate 1&2 (re-do)

Fragment Analysis Submission

  • combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)


20130225 Frag.png


20130225 FedEx.png


  • UAGC Submission# XPTR & VMZX


  • FedEx picked up 2:41pm, 25 February
  • FedEx delivered 9:12am, 26 February
  • UAGC received 10:43am, 26 February
  • UAGC completed 9:28am, 27 February


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


PCR

  • 20130225 PCRa.png


  • 20130225 PCRb.png