Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09"

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Line 9: Line 9:
  
  
==CNTI Cvanµ23==
+
==ASHY Cvanµ23==
  
  
Line 26: Line 26:
 
[[Image:20130209_Gela.png|800 px]]
 
[[Image:20130209_Gela.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
==CNTI Cvanµ24==
+
==ASHY Cvanµ24==
  
  
Line 52: Line 50:
 
[[Image:20130209_Gelb.png|800 px]]
 
[[Image:20130209_Gelb.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
+
* it works! Yay! Many possibilities
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
==CNTI Cvanµ26==
+
==ASHY Cvanµ26==
  
  
Line 78: Line 74:
 
[[Image:20130209_Gelc.png|800 px]]
 
[[Image:20130209_Gelc.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  
==CNTI Cvanµ27==
+
==ASHY Cvanµ27==
  
  
Line 104: Line 98:
 
[[Image:20130209_Geld.png|800 px]]
 
[[Image:20130209_Geld.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
  

Revision as of 14:43, 12 February 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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ASHY Cvanµ23

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gela.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ24

PCR

  • 20130209 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gelb.png

  • it works! Yay! Many possibilities


ASHY Cvanµ26

PCR

  • 20130209 PCRb.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gelc.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ27

PCR

  • 20130209 PCRc.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Geld.png

  • mispriming everywhere - primer pair not usable for ASHY