Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08"

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(Autocreate 2013/02/08 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
+
==CNTI Cvanµ18==
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20130208_Gela.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for CNHY
 +
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 +
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 +
 
 +
 
 +
==CNTI Cvanµ19==
  
  
 
===PCR===
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
+
* [[Image:20130208_PCRa.png|800 px]]
  
  
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[[Image:20120110_Gela.png|800 px]]
+
[[Image:20130208_Gelb.png|800 px]]
  
 
* mispriming everywhere - primer pair not usable for CNHY
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 +
 +
 +
==CNTI Cvanµ23==
 +
 +
 +
===PCR===
 +
* [[Image:20130208_PCRb.png|800 px]]
 +
 +
 +
 +
 +
 +
  
  

Revision as of 13:12, 12 February 2013

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CNTI Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png


CNTI Cvanµ19

PCR

  • 20130208 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gelb.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png


CNTI Cvanµ23

PCR

  • 20130208 PCRb.png