User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07: Difference between revisions

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==CNTI Cvanµ4==
==ASHY Cvanµ4==




Line 30: Line 30:
[[Image:20130207_Gela.png|800 px]]
[[Image:20130207_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ7==
==ASHY Cvanµ7==




Line 56: Line 54:
[[Image:20130207_Gelb.png|800 px]]
[[Image:20130207_Gelb.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ18==
==ASHY Cvanµ18==




Revision as of 14:36, 12 February 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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ASHY Cvanµ4

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ7

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ18

PCR



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