User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07

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ASHY Cvanµ4

PCR

  • 20130207 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130207 Gela.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ7

PCR

  • 20130207 PCRb.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130207 Gelb.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ18

PCR

  • 20130207 PCRc.png