User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07: Difference between revisions

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(Autocreate 2013/02/07 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
==CNTI Cvanµ4==




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130207_PCRa.png|800 px]]




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[[Image:20120110_Gela.png|800 px]]
[[Image:20130207_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for CNHY
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


==CNTI Cvanµ7==
===PCR===
* [[Image:20130207_PCRb.png|800 px]]
===Gel===
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
[[Image:20130207_Gelb.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
==CNTI Cvanµ18==
===PCR===
* [[Image:20130207_PCRc.png|800 px]]




Revision as of 14:10, 12 February 2013

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CNTI Cvanµ4

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


CNTI Cvanµ7

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


CNTI Cvanµ18

PCR



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