User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27: Difference between revisions
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* CNHY MP1a: decrease Acos5 | * CNHY MP1a: decrease Acos5 | ||
* CNHY MP1b: increase Ai5057, Ai5062 | * CNHY MP1b: increase Ai5057, Ai5062 | ||
===Frag Results=== | ===Frag Results=== | ||
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====MP1a==== | ====MP1a==== | ||
= | |||
{| {{table}} style="text-align: center" | |||
: still fine, though seems to decrease with increasing Acos5 | |- | ||
= | ! !! 23 July 2012 !! this time | ||
|- | |||
| style="background: blue" | Ai5043 || did not amplify || did not amplify; '''remove from multiplex''' | |||
|- | |||
| style="background: green" | Acos3 || amplified well; no changes needed || still fine, though seems to decrease with increasing Acos5 & Ai5071 | |||
|- | |||
= | | style="background: yellow" | Acos5 || amplified with high intensity, pull down a bit || 0.04 = too low; 0.06 = inconclusive (failures); 0.08 = too high | ||
|- | |||
| style="background: yellow" | Ai5013 || amplified with very low intensity; increase concentration || second peak never got above 250, regardless of concentration; but in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased | |||
|- | |||
| style="background: red" | Ai5071 || amplified with lower intensity; increase a bit || best at 0.24, 0.28, 0.32 (200-300 intensity only) | |||
= | |} | ||
* since removing the only marker labelled with FAM, move one of the NEDs to FAM | |||
* NEDs: Acos5 and Ai5013 ... since Ai5013 tends to be low intensity, would be a good one to move to brighter FAM | |||
====MP1b==== | ====MP1b==== | ||
{| {{table}} style="text-align: center" | |||
|- | |||
! !! 23 July 2012 !! this time | |||
|- | |||
| style="background: blue" | Ai5033 || amplified well; no changes needed || still fine (no amp for any markers in Ai5057 assay) | |||
|- | |||
| style="background: blue" | Ai5057 || did not amplify || did not amplify; '''remove from multiplex''' | |||
|- | |||
| style="background: green" | Ai5037 || amplified well; no changes needed || still fine (no amp for any markers in Ai5057 assay) | |||
|- | |||
| style="background: yellow" | Ai5035 || amplified well; no changes needed || a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay) | |||
|- | |||
| style="background: red" | Ai5062 || amplified but with very low intensity; increase concentration || best at 0.48 (400 & 6000 intensity) | |||
|} | |||
Revision as of 14:48, 5 December 2012
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CNHY MP1a/b Optimization
Frag Results
MP1a
MP1b
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