Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15"

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(Autocreate 2012/11/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
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==SCOC Batch03==
 +
* Samples = USGS TP & CPN + Clark TP & SYR
  
  
===PCR===
+
===Gel===
* [[Image:20120110_PCRa.png|800 px]]
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* [[Image:20121108_Gel.png|800 px]]
 +
 
 +
 
 +
* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
 +
 
 +
 
 +
===Fragment Analysis Submission===
 +
 
  
 +
* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
  
===Gel===
 
  
{| {{table}} style="text-align: center"
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:: [[Image:20121108_Frag.png|900 px]]
|-
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! Pour !! Load !! Run
+
 
|-
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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:: [[Image:20121108_FedEx.png|700 px]]
|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
  
 +
* UAGC Submission# TZNF & WKAY
  
[[Image:20120110_Gela.png|800 px]]
 
  
* mispriming everywhere - primer pair not usable for CNHY
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* FedEx picked up 2:48pm, 8 November
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* FedEx delivered 9:16am, 9 November
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* UAGC received 10:55am, 9 November
 +
* UAGC completed 1:54pm, 9 November
  
  

Revision as of 15:21, 15 November 2012

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SCOC Batch03

  • Samples = USGS TP & CPN + Clark TP & SYR


Gel

  • 20121108 Gel.png


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.


Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y


20121108 Frag.png


20121108 FedEx.png


  • UAGC Submission# TZNF & WKAY


  • FedEx picked up 2:48pm, 8 November
  • FedEx delivered 9:16am, 9 November
  • UAGC received 10:55am, 9 November
  • UAGC completed 1:54pm, 9 November