Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25"

From OpenWetWare
Jump to: navigation, search
Line 10: Line 10:
  
 
==SCOC Batch 2==
 
==SCOC Batch 2==
 +
* Samples = USGS SYR & LOM + Clark HOL & RAJ
  
  
 
===Gel===
 
===Gel===
 +
* [[Image:20121025_Gel.png|800 px]]
  
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
[[Image:20120110_Gela.png|800 px]]
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
 +
* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
  
  

Revision as of 16:00, 25 October 2012

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC Batch 2

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


Gel

  • 20121025 Gel.png


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.