User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25: Difference between revisions

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==SCOC Batch 2==
==SCOC Batch 2==
* Samples = USGS SYR & LOM + Clark HOL & RAJ




===Gel===
===Gel===
* [[Image:20121025_Gel.png|800 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.




Revision as of 16:00, 25 October 2012

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SCOC Batch 2

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


Gel


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.



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