Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25"

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(Autocreate 2012/10/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
(2 intermediate revisions by the same user not shown)
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==CNTI Acos5==
+
==SCOC Batch02==
 
+
* Samples = USGS SYR & LOM + Clark HOL & RAJ
 
 
===PCR===
 
* [[Image:20120110_PCRa.png|800 px]]
 
  
  
 
===Gel===
 
===Gel===
 +
* [[Image:20121025_Gel.png|800 px]]
  
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
[[Image:20120110_Gela.png|800 px]]
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
  
 +
* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
  
  

Revision as of 08:43, 26 October 2012

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SCOC Batch02

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


Gel

  • 20121025 Gel.png


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.