Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2012/10/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Line 9: Line 9:
==CNTI Acos5==
==SCOC Batch 2==
* [[Image:20120110_PCRa.png|800 px]]

Revision as of 15:19, 25 October 2012

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

SCOC Batch 2


Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes

20120110 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png