User:Tara K. Luckau/Notebook/Team ConGen/2012/01/14

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CNHY / CNTI Multiplex

PCR - Uniplexes

  • uniplex to determine marker range
  • individual CNHY and CNTI DNAs to determine marker quality when uniplexed
  • pooled CNHY and CNTI DNAs to determine marker range when uniplexed


PCR - Multiplex all-in-one

  • multiplex to determine general marker quality and strength
  • individual CNHY and CNTI DNAs to determine marker quality when multiplexed
  • pooled CNHY and CNTI DNAs to determine marker range when multiplexed


PCR - Multiplex A and Multiplex B

  • split multiplex into two (no post-PCR mixing) based on primer interactions, gradient results, and estimated amplicon size
  • compare all-in-one multiplex with Multiplex A and B - better?
  • compare scores in multiplex A and B with uniplex to make sure scores match and nothing weird is happening





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