User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

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CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...

Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
  • 20120106 GradientSummary.png

Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
  • 20120106 Map.png


  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
  • 20120106 AutoDimer.png

PCR - determine approximate range of each marker

  • 20120106 PCR.png