User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
(5 intermediate revisions by the same user not shown) | |||
Line 9: | Line 9: | ||
== | ==ASHY Multiplex== | ||
* prepping to start multiplexing CNHY primers ... so see primer interactions ... | * prepping to start multiplexing CNHY primers ... so see primer interactions ... | ||
===Gradient Summary=== | |||
* green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary | |||
* [[Image:20120106 GradientSummary.png]] | |||
===Multiplex Map=== | |||
* markers placed arbitrarily | |||
* marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel. | |||
* [[Image:20120106 Map.png]] | |||
===AutoDimer=== | ===AutoDimer=== | ||
* color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions | |||
* [[Image:20120106_AutoDimer.png|800 px]] | * [[Image:20120106_AutoDimer.png|800 px]] | ||
===PCR - determine approximate range of each marker=== | |||
* pool high-quant DNA samples from throughout SD county: | |||
:-- CNHY CPN 02 | |||
:-- CNHY LOM 08 | |||
:-- CNHY RAJ 01 | |||
:-- CNHY RAJ 35 | |||
:-- CNHY RAJ 42 | |||
:-- CNHY RAJ 50 | |||
:-- CNHY TP1 11 | |||
:-- CNHY TP3 05 | |||
:-- CNHY TP3 19 | |||
:-- CNHY TP3 20 | |||
* 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL) | |||
* [[Image:20120106_PCR.png|800 px]] | |||
Revision as of 09:40, 20 February 2013
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ASHY Multiplex
Gradient Summary
Multiplex Map
AutoDimer
PCR - determine approximate range of each marker
|