Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06"

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* color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
 
* color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
 
* [[Image:20120106_AutoDimer.png|800 px]]
 
* [[Image:20120106_AutoDimer.png|800 px]]
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 +
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===PCR - determine approximate ranges of each marker===
 +
* [[Image:20120106_PCR.png|800 px]]
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Revision as of 14:09, 6 January 2012

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
  • 20120106 GradientSummary.png


Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
  • 20120106 Map.png


AutoDimer

  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
  • 20120106 AutoDimer.png


PCR - determine approximate ranges of each marker

  • 20120106 PCR.png