Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06"

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(Autocreate 2012/01/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNHY Acos5==
+
==CNHY Multiplex==
  
  
===PCR===
+
* prepping to start multiplexing CNHY primers ... so see primer interactions ...
* [[Image:20111023_PCRa.png|800 px]]
 
  
  
===Gel===
+
===AutoDimer===
 
+
* [[Image:20120106_AutoDimer.png|800 px]]
{| {{table}} style="text-align: center"
 
|-
 
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
|}
 
 
 
 
 
[[Image:20111023_Gel.png|800 px]]
 
 
 
* mispriming everywhere - primer pair not usable for CNHY
 
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
 
 
  
  

Revision as of 13:46, 6 January 2012

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CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


AutoDimer

  • 20120106 AutoDimer.png