Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2010/12/03"

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(Failed Gel on Scun22)
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
 
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
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Revision as of 12:28, 12 January 2011

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Failed Gel on Scun22

  • power supply started smoking ...
  • found different power supply, but no bubbles
  • borrowed a rig from Bohonak's lab, but not very many bubbles
  • made new TAE buffer (had been re-using the buffer, this was approx the 5th gel), but no dye migration
  • made new agarose gel from new TAE buffer with ladder and dye, ran on borrowed rig with our power supply, nada
  • brought rig with gel and buffer to Bohonak's power supply - dye migration within 5 minutes.

Conclusion: find a new power supply that works.

Ugh.