User:Tara K. Luckau/Notebook/Team ConGen/2010/10/11

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PCR Thermalcycler Testing

First Attempt

Description

  • 10μL in each well of full-skirt PCR plate, PCR seal
  • First set-up of thermalcycler, established User, Protocols, Plates and Masters
  • Data: 20101011_TKL_CyclerTest.tad

Results

  • Evaporation all around edges
  • Try using silicone mat?

1 Mat

Description

  • 10μL in each well of full-skirt PCR plate, PCR seal, 1 silicone mat
  • Data: 20101011_TKL_CyclerTest1Mat.tad

Results

  • Still evaporation around edges, but better
  • Try using 2 silicone mats?