Difference between revisions of "User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/20"

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(Autocreate 2013/01/20 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing)
 
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==Entry title==
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==Running Gels==
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* I already have some 1% gel made up, so I am going to try running the samples on it using the big gel bath. If if doesn't work, I am going to try 2% on the little bath. I am going to use 3ul of DNA/buffer per well to maximise the amount of samples I can run and compare.
  
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1ul buffer to 5ul DNA, 2ul buffer to 10ul DNA, 4ul buffer to 20ul DNA.
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I think I will make up 4ul buffer and then add 20ul of DNA. This should allow me to run the samples 6 times.
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no visible results. Try again on smaller gel
  
 
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Revision as of 06:13, 20 January 2013

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Running Gels

  • I already have some 1% gel made up, so I am going to try running the samples on it using the big gel bath. If if doesn't work, I am going to try 2% on the little bath. I am going to use 3ul of DNA/buffer per well to maximise the amount of samples I can run and compare.

1ul buffer to 5ul DNA, 2ul buffer to 10ul DNA, 4ul buffer to 20ul DNA. I think I will make up 4ul buffer and then add 20ul of DNA. This should allow me to run the samples 6 times.

no visible results. Try again on smaller gel