Difference between revisions of "User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Results==
* Insert content here...
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* last samples produced valid results when compared with positive controls
  
 +
Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.
 +
 +
Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.
 +
 +
Master Mix:
 +
7 x lorikeets
 +
2 x positive controls (male female)
 +
1 negative control
 +
 +
*1ul F primer
 +
*1ul R primer
 +
*2.0ul dNTPs
 +
*0.5u Taq
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*1ul DNA
 +
*2.5ul 10x buffer
 +
*1.5ul MgCl<sub>2</sub>
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*16ul H<sub>2</sub>O
 +
 +
*1:1SWWBD
 +
*2:2SWWBD
 +
*3:R5#9
 +
*4:R5#51
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*5:R5#52
 +
*6:R5#100
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*7 :Pink Gold
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male
 +
female
 +
neg
 +
 +
Results: no bands. Try lower annealing temperature - say 55°C
  
 
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Latest revision as of 22:21, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Results

  • last samples produced valid results when compared with positive controls

Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.

Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg

Results: no bands. Try lower annealing temperature - say 55°C