Difference between revisions of "User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09"

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(Entry title)
(Bird PCR)
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Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.
 
Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.
  
Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples.
+
Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples.
  
 
Master Mix:
 
Master Mix:
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1 negative control
 
1 negative control
  
20ul total volume
+
*1ul F primer
 +
*1ul R primer
 +
*2.0ul dNTPs
 +
*0.5u Taq
 +
*1ul DNA
 +
*2.5ul 10x buffer
 +
*1.5ul MgCl<sub>2</sub>
 +
*16ul H<sub>2</sub>O
  
 +
*1:1SWWBD
 +
*2:2SWWBD
 +
*3:R5#9
 +
*4:R5#51
 +
*5:R5#52
 +
*6:R5#100
 +
*7 :Pink Gold
 +
male
 +
female
 +
neg
  
 
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Revision as of 16:25, 8 January 2013

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Bird PCR

Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.

Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg