Difference between revisions of "User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09"

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(Autocreate 2013/01/09 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing)
 
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==Entry title==
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==Bird PCR==
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Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.
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Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples.
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Master Mix:
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7 x lorikeets
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2 x positive controls (male female)
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1 negative control
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20ul total volume
  
  

Revision as of 16:06, 8 January 2013

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Bird PCR

Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.

Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

20ul total volume