User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04

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PCR with 2550/2178 primer pair at 60°C

Aim: see if these primers are better at sex differentiation than the p2/p8 ones

method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday

NB the 94°C soak went for 8.30 instead of 2 mins.

Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.