User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/03
|Project name||<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page|
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
Visualising lori's p2/p8 on 1% gel
Aim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel.
Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily.
Results: Gels can be seen here: 
It looks as though the samples A and B are male and that D is female, with the rest being ambiguous.
Aim - try to see if results are better at 60°C
Follwing exact same protocol as here:  Master mix for all 8 samples plus -ve control(10 units of MM):
Tubes labelled as follows:
PCR the same except annealing temp is 60°C (user 77 file 81).
results to be determined via agar gel E.Phoresis
Same as above, samples AT,BT..etc
PCR anneal at 55°C