User:Stuart McKellar/Notebook/Bird Sex Testing/2012/11/13: Difference between revisions
From OpenWetWare
(Autocreate 2012/11/13 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing) |
(fix raw html notebook nav) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==gel electrophoresis== | ||
* | * The aim of this lab is to determine the sex of the rainbow lorikeets through agar gel electrophoresis. Gel is 2% agar and stained with GelGreen that has been sitting on my desk room temperature since last test (which was successful). I will run a ladder as well to make sure that the gel is still showing luminescence. | ||
If the PCR failed, I expect the DNA not to migrate. I am loading 5ul DNA sample with 1ul x 6x DNA running buffer (glycerol + bromophenol blue). | |||
Samples were loaded as follows: | |||
*1SWWBD | |||
*2SWWBD | |||
*R5#9 | |||
*Ladder (300-10KB) | |||
*R5#51 | |||
*R5#52 | |||
*R5#100 | |||
*Pink Gold | |||
Gel ran at 100V (intially started at 300V for a few seconds and then pansied out) and 60mA. Immediate illumination could be seen in the well with the ladder. Gel is running and I can see the ladder but nothing else. Fuck. Looks like I am going back to PCR. After 300bp had reached centre, still no light from samples. Maybe run a single lorikeet sample through PCR and then check on a gel. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Latest revision as of 22:14, 26 September 2017
 Project name
|
 Main project page Previous entry Next entry
|
gel electrophoresis
If the PCR failed, I expect the DNA not to migrate. I am loading 5ul DNA sample with 1ul x 6x DNA running buffer (glycerol + bromophenol blue). Samples were loaded as follows:
Gel ran at 100V (intially started at 300V for a few seconds and then pansied out) and 60mA. Immediate illumination could be seen in the well with the ladder. Gel is running and I can see the ladder but nothing else. Fuck. Looks like I am going back to PCR. After 300bp had reached centre, still no light from samples. Maybe run a single lorikeet sample through PCR and then check on a gel. | |
