# Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30"

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

## BSA and HAuCl4 Stock Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.21mg
• therefore, I need 12.07mL H2O
• BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 21.5mg BSA
• therefore, I need 21.47mL of H2O

## 20mL Hydrogels 130K PVA; Various Ratios

• Ratio 1: 60 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00417mM
• solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
• Ratio 2: 90 Au:BSA (2 dishes of this ratio)
• fixed: .25mM Au
• new BSA: 0.00278mM
• solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
• Ratio 3: 120 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00208mM
• solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
• Ratio 4: 150 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00167mM
• solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
• All 5 solutions put into the 80°C oven at