# Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26"

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## Autoclaving PVA + Au:BSA Solutions

• Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
• Tube 1: 0.6g PVA 89-98K + 10mL water
• add 1mL Gold + 1mL BSA
• Tube 2: 0.6g PVA 130K + 10mL water
• add 1mL Gold + 1mL BSA
• Tube 3: 0.25mM Gold + 0.00147mM BSA + water
• Tube 4: 0.25mM Gold + 0.00278mM BSA + water
• Tube 5: 0.25mM Gold + 0.00147mM BSA + water
• Tube 6: 0.25mM Gold + 0.00278mM BSA + water
• Tube 7: nothing
• add 1mL Gold + 1mL BSA + 8mL water
• Tube 8: nothing
• add 1mL Gold + 1mL BSA + 8mL water
• All solutions put into the autoclave at 12:05PM

## BSA and HAuCl4 Stock Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.21mg
• therefore, I need 12.07mL H2O
• BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 21.5mg BSA
• therefore, I need 21.47mL of H2O

## 20mL Gold:BSA solutions, 60/90/120/150 Ratios

• Ratio 1: 60 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00417mM
• solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
• Ratio 2: 90 Au:BSA (2 dishes of this ratio)
• fixed: .25mM Au
• new BSA: 0.00278mM
• solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
• Ratio 3: 120 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00208mM
• solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
• Ratio 4: 150 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00167mM
• solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
• All 5 solutions put into the 80°C oven at 1:22PM and taken out at 5:20PM.
• Added 0.6g 89-98K PVA to each solution; autoclaved for an hour.