# Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26"

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## Autoclaving PVA + Au:BSA Solutions

• Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
• Tube 1: 0.6g PVA 89-98K + 10mL water
• add 1mL Gold + 1mL BSA
• Tube 2: 0.6g PVA 130K + 10mL water
• add 1mL Gold + 1mL BSA
• Tube 3: 0.25mM Gold + 0.00147mM BSA + water
• Tube 4: 0.25mM Gold + 0.00278mM BSA + water
• Tube 5: 0.25mM Gold + 0.00147mM BSA + water
• Tube 6: 0.25mM Gold + 0.00278mM BSA + water
• Tube 7: nothing
• add 1mL Gold + 1mL BSA + 8mL water
• Tube 8: nothing
• add 1mL Gold + 1mL BSA + 8mL water
• All solutions put into the autoclave at 12:05PM

## BSA and HAuCl4 Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.21mg
• therefore, I need 11.224mL H2O
• BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 20.24mg BSA
• therefore, I need 20.208mL of H2O