Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09"

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(BSA and HAuCl4 Solutions)
(BSA and HAuCl4 Solutions)
Line 11: Line 11:
 
**MW = 393.83 g/mol
 
**MW = 393.83 g/mol
 
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
 
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
 +
***actual amount used: 10.01mg
 +
***therefore, I need 11.0048mL H<sub>2</sub>O
 
*BSA solution
 
*BSA solution
 
**MW = 66776g
 
**MW = 66776g
**in 10mL 15μM solution, I need 10.016mg BSA
+
**in 20mL 15μM solution, I need 20.032mg BSA
 +
***actual amount used: 20.120mg BSA
 +
***therefore, I need 20.0879mL of H<sub>2</sub>O
  
  

Revision as of 07:03, 9 July 2013

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BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O