User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
| (6 intermediate revisions by one other user not shown) | |||
| Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
| Line 11: | Line 11: | ||
**MW = 393.83 g/mol | **MW = 393.83 g/mol | ||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | **in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | ||
*BSA solution | ***actual amount used: 10.01mg | ||
***therefore, I need 11.0048mL H<sub>2</sub>O | |||
*89-98,000 BSA solution | |||
**MW = 66776g | **MW = 66776g | ||
**in | **in 20mL 15μM solution, I need 20.032mg BSA | ||
***actual amount used: 20.120mg BSA | |||
***therefore, I need 20.0879mL of H<sub>2</sub>O | |||
==Gold:BSA ratios== | |||
*Ratio 1: 60 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00417mM | |||
**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H<sub>2</sub>O | |||
*Ratio 2: 90 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00278mM | |||
**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H<sub>2</sub>O | |||
*Ratio 3: 120 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00208mM | |||
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H<sub>2</sub>O | |||
*Ratio 4: 150 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00167mM | |||
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O | |||
*All solutions put into the 80°C oven at 12:07PM and taken out at 4PM | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Latest revision as of 23:03, 26 September 2017
 Project name
|
 Main project page Previous entry Next entry
|
BSA and HAuCl4 Solutions
Gold:BSA ratios
| |
