Difference between revisions of "User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/07/09 Entry for User:Stephanie_Tietz/Notebook/Stephanies_Notebook)
 
(fix raw html notebook nav)
 
(10 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==BSA and HAuCl<sub>4</sub> Solutions==
* Insert content here...
 
  
 +
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
 +
**MW = 393.83 g/mol
 +
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
 +
***actual amount used: 10.01mg
 +
***therefore, I need 11.0048mL H<sub>2</sub>O
 +
*89-98,000 BSA solution
 +
**MW = 66776g
 +
**in 20mL 15μM solution, I need 20.032mg BSA
 +
***actual amount used: 20.120mg BSA
 +
***therefore, I need 20.0879mL of H<sub>2</sub>O
 +
 +
==Gold:BSA ratios==
 +
*Ratio 1: 60 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00417mM
 +
**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H<sub>2</sub>O
 +
*Ratio 2: 90 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00278mM
 +
**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H<sub>2</sub>O
 +
*Ratio 3: 120 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00208mM
 +
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H<sub>2</sub>O
 +
*Ratio 4: 150 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00167mM
 +
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O
 +
*All solutions put into the 80°C oven at 12:07PM and taken out at 4PM
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 23:03, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • 89-98,000 BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O

Gold:BSA ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
  • All solutions put into the 80°C oven at 12:07PM and taken out at 4PM