Difference between revisions of "User:Sim Huay Ping/Notebook/CBE/08/150/2009/01/13"

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(Entry title)
(AFM sample preparation)
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To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated
 
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated
 +
 
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated
 
To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated
 +
 
Put both microvial into the shaking incubate and leave for 15 mins
 
Put both microvial into the shaking incubate and leave for 15 mins
 +
 
Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl
 
Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl
 +
 
To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge
 
To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge
 +
 
To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge
 
To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge
 +
 
Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other
 
Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other
 +
 
Leave the sample to dry for 15 mins
 
Leave the sample to dry for 15 mins
  

Revision as of 08:04, 4 February 2009

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AFM sample preparation

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and label as untreated

To 100ml of bacteria innoculum in a microvial, add 100ul of AMP and 6ul of AMP and label as treated

Put both microvial into the shaking incubate and leave for 15 mins

Wash, centrifuge and vortex the microvials 3 times with 0.9% NaCl

To the pellet after washing, add in 500ul of 50% ethanol and then vortex and centrifuge

To the pellet after washing with 50% ethanol, add in 500ul of 90% ethanol and then vortex and centrifuge

Pipette 10ul of each treated and the untreated mixture onto the microscope glass. Spread the samples beside each other

Leave the sample to dry for 15 mins