Difference between revisions of "User:Sim Huay Ping/Notebook/CBE/08/150/2009/01/08"

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(Entry title)
(Membrane Depolarisation)
Line 9: Line 9:
  
 
Performed membrane depolarisation experiment on zhou's AMP
 
Performed membrane depolarisation experiment on zhou's AMP
 +
 
Performed experiment on Ecoli, Pseudomonas Aeruginosa, Serratia and Candida.
 
Performed experiment on Ecoli, Pseudomonas Aeruginosa, Serratia and Candida.
The first peak of depolarisation did not occur, instead the fluorescene emission increased exponentially without decreasing. After Gramicidin was added at 130 seconds, the fluorescence increased only slightly.
+
 
 +
The first peak of depolarisation did not occur, instead the fluorescene emission increased  
 +
 
 +
exponentially without decreasing. After Gramicidin was added at 130 seconds, the fluorescence  
 +
 
 +
increased only slightly.
 +
 
 
Saved results as
 
Saved results as
 +
 
zhou_ecoli1
 
zhou_ecoli1
 +
 
zhou_ecoli2
 
zhou_ecoli2
 +
 
zhou_pseudo1
 
zhou_pseudo1
 +
 
zhou_serratia8jan1
 
zhou_serratia8jan1
 +
 
zhou_ecoli8jan1
 
zhou_ecoli8jan1
 +
 
zhou_candida8jan1
 
zhou_candida8jan1
 +
 
zhou_candida8jan2
 
zhou_candida8jan2
 +
 
zhou_pseudo8jan1
 
zhou_pseudo8jan1
 +
 
Will attempt to reduce the amount of dye used at the next experimental trial
 
Will attempt to reduce the amount of dye used at the next experimental trial
  

Revision as of 05:28, 4 February 2009

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Membrane Depolarisation

Performed membrane depolarisation experiment on zhou's AMP

Performed experiment on Ecoli, Pseudomonas Aeruginosa, Serratia and Candida.

The first peak of depolarisation did not occur, instead the fluorescene emission increased

exponentially without decreasing. After Gramicidin was added at 130 seconds, the fluorescence

increased only slightly.

Saved results as

zhou_ecoli1

zhou_ecoli2

zhou_pseudo1

zhou_serratia8jan1

zhou_ecoli8jan1

zhou_candida8jan1

zhou_candida8jan2

zhou_pseudo8jan1

Will attempt to reduce the amount of dye used at the next experimental trial