Difference between revisions of "User:Sim Huay Ping/Notebook/CBE/08/150/2008/12/11"

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(Autocreate 2008/12/11 Entry for User:Sim_Huay_Ping/Notebook/CBE/08/150)
 
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==Entry title==
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==Kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP==
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Extracted 500ul of Pseudomonas inoculum left overnight into 5ml of nutrient broth for regeneration
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Left the Pseudomonas inoculums in the incubator for 4 hours
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Transferred 1000ul of Pseudomonas inoculums into vials
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Centrifuged, washed and vortex 3 times with PBS and resuspended the pellet in 1000ul of PBS
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Used the UV spectrometer to measure the inoculum sample to 0.05
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Performed kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP
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Extracted single colony from bacteria streak plates and transferred the single colony into 5ml of nutrient broth
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Left the bacteria inoculums in the incubator overnight
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Kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP

Extracted 500ul of Pseudomonas inoculum left overnight into 5ml of nutrient broth for regeneration

Left the Pseudomonas inoculums in the incubator for 4 hours

Transferred 1000ul of Pseudomonas inoculums into vials

Centrifuged, washed and vortex 3 times with PBS and resuspended the pellet in 1000ul of PBS

Used the UV spectrometer to measure the inoculum sample to 0.05

Performed kinetics experiment on Pseudomonas, using 6.2ul of Zhou’s AMP


Extracted single colony from bacteria streak plates and transferred the single colony into 5ml of nutrient broth

Left the bacteria inoculums in the incubator overnight