Difference between revisions of "User:ShLo"

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Stephanie Lo <br>
Harvard College 2010
c/o Harvard College PRISE<br>
Leverett Mail Center<br>
28 DeWolfe Street<br>
Cambridge, MA 02138
I have always had a passion for sciences, particularly chemistry, and hope to pursue a joint concentration in Molecular and Cellular Biology and Economics. Eventually, I plan to apply for an MD/PhD program and specialize in digestive disorders.
NanoDrop results (6/20) performed by Ellenor and Stephanie:<br>
(1.5 uL used out of a 30uL elution with nuclease free water)<br>
S: 10.9 ng/uL<br>
S2: 15.4 ng/uL<br>
B: 59.1 ng/uL<br>
B1: 25.1 ng/uL<br>
Brainstorming for the two-component systems (really for my own use for now - not expected to be coherent)<br>
[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=10653699&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus Structural comparison of the PhoB and OmpR DNA binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box]<br>
-NMR used to determine 3DE structure of PhoB DNA-binding/transactivation domain. Very similar to OmpR DNA-binding/transactivation domain, except for conformation of the long turn region of PhoB (interaction site for sigma subunit, rather than interaction with alpha subunit for OmpR)
[http://jb.asm.org.ezp1.harvard.edu/cgi/content/full/185/1/317?view=long&pmid=12486069 Interdomain linkers of homologous response regulators determine their mechanism of action]<br>
Focuses on OmpR and PhoB and, as the title suggests, supports that phosphorylation of sites (particularly N-terminus of both proteins) improves affinity to bind DNA. Isolated C terminus of OmpR is insufficient to productively interact with RNA polymerase. <br>
I've been told by some of the lab members that OmpR is an inner-membrane protein and therefore cannot be used for our assays. It seems that we'll have to find another protein ...<br>
[http://openwetware.org/wiki/IGEM:Harvard/2007 Harvard iGEM]

Latest revision as of 08:48, 12 July 2007

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