User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/27: Difference between revisions
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* For there to be 0.5 μg of DNA to digest in 10 μL, which is enough to see on a gel, I'll need 50 ng/μL ~ 15 nM final concentration DNA. | * For there to be 0.5 μg of DNA to digest in 10 μL, which is enough to see on a gel, I'll need 50 ng/μL ~ 15 nM final concentration DNA. | ||
* 50 μL WP-PCR reaction w/o primers: | * 50 μL WP-PCR reaction w/o primers: | ||
** | ** 36 μL H<sub>2</sub>O | ||
** 7.5 μL 100 nM ΦX174 template (~15 nM final) | ** 7.5 μL 100 nM ΦX174 template (~15 nM final) | ||
** 5 μL 10X reaction buffer | ** 5 μL 10X reaction buffer |
Revision as of 11:52, 28 September 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Hypothesis 2: Gene L is necessary for phage propagation.
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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