User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/27: Difference between revisions
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===Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.=== | ===Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.=== | ||
* Graphs of MGapt and deGFP vs. time from yesterday's experiment are simply beautiful. | * [http://openwetware.org/images/1/17/Expression_results_092612.pdf Graphs of MGapt and deGFP vs. time from yesterday's experiment] are simply beautiful. | ||
* However, the data is very noisy at the 1.11 nM level or below, even though the deGFP graphs show that expression is occurring down to at least the .37 nM level. | * However, the data is very noisy at the 1.11 nM level or below, even though the deGFP graphs show that expression is occurring down to at least the .37 nM level. | ||
* Therefore, I will do a range of malachite green at 1 nM, to try and improve the signal at lower plasmid concentrations. | * Therefore, I will do a range of malachite green at 1 nM, to try and improve the signal at lower plasmid concentrations. |
Revision as of 10:22, 28 September 2012
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Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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