Difference between revisions of "User:Sean E. Lauber"

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==Contact Info==
 
==Contact Info==
  
*s_laub@live.concordia.ca
+
*sean.laub@gmail.com
 +
 
 
==Education==
 
==Education==
 +
*M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
 +
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
 +
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
 +
 +
==Protocols==
 +
 +
====Richards Lab Protocols====
  
*B.Sc, Concordia (Montreal) 2010
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=====Molecular Techniques=====
 +
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
 +
*[[Sean_Lauber:ELISA Development| ELISA Development]]
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*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
 +
*[[Sean_Lauber:Generating Adenovirus Lysate | Generating Adenovirus Lysate]]
 +
*[[Sean_Lauber:PCR| PCR]]
 +
*[[Sean_Lauber:Stable Transfection Using Lipofectamine 2000| Stable Transfection Using Lipofectamine 2000]]
 +
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
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*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
 +
*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]]
 +
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
 +
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
  
==Research interests==
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=====Mammalian Cell Culture=====
 +
*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]]
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*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]]
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*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
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*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
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*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
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*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]
  
==Publications==
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=====Technical=====
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*[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]]
 +
*[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]]
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*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
 +
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
 +
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
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*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
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*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
 +
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]
  
One day...
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=====Animal Work=====
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*[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
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*[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]]
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*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
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*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
 +
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Using the Kras G12D Mouse Model for Lung Cancer]]
 +
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer]]
 +
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
 +
*[[Sean_Lauber:Differential cell staining|Differential cell staining]]
  
==My brilliant contributions==
+
====Joyce Lab Protocols==== 
  
====Joyce May-Dec 2009==== 
 
[[Joyce: Project overview| Project overview May-Dec 2009]]
 
  
 
*[[Joyce:DNA electrophoresis| DNA Electrophoresis]]
 
*[[Joyce:DNA electrophoresis| DNA Electrophoresis]]
*[[Joyce:Transformation| Transformation]]
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*[[Joyce:Transformation| Bacterial Transformation]]
 
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]]
 
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]]
 
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]]
 
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]]
*[[Joyce: Phenol extraction| Phenol extraction]]
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*[[Joyce: Phenol extraction| DNA Phenol extraction]]
 
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]]
 
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]]
 
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
 
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Ligation|Ligation]]
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*[[Joyce: Ligation| DNA Ligation]]
 
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*[[Sean_Lauber: PCR Mutagenesis| PCR Mutagenesis]]
====Piekny Sept - 2009====
 
 
 
*[[Piekny: splitting cells|Splitting cells]]
 
  
 
==Links==
 
==Links==
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*[http://www.fermentas.com/techinfo/re/troubleshoot.htm Restriction digest troubleshooting]
 
*[http://www.fermentas.com/techinfo/re/troubleshoot.htm Restriction digest troubleshooting]
 
*[http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html More troubleshooting]
 
*[http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html More troubleshooting]
 +
*[http://www.jove.com/ Journal of Visualized Experiments]
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*[http://psort.ims.u-tokyo.ac.jp/ PSORT]
  
====Fun stuff, useful links====
+
====Useful links====
  
 
*[http://bitesizebio.com/ Bitesize Bio]
 
*[http://bitesizebio.com/ Bitesize Bio]
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inheritance in Man]
 
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inheritance in Man]
 
*[http://www.ncbi.nlm.nih.gov/ NCBI]
 
*[http://www.ncbi.nlm.nih.gov/ NCBI]
 +
*[http://www.uniprot.org/ Uniprot (as good as NCBI)]
 
*[http://tools.neb.com/NEBcutter2/ Nebcutter 2.0]
 
*[http://tools.neb.com/NEBcutter2/ Nebcutter 2.0]
*[http://www.fruitfly.org/seq_tools/promoter.html/ Promoter Predictor]
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*[http://www.vivo.colostate.edu/molkit/translate/ Translate DNA]
 +
*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
 
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
 
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
 
*[https://mozy.com/home Backup your data with Mozy]
 
*[https://mozy.com/home Backup your data with Mozy]
 +
*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)]
  
 
====Openwetware related====
 
====Openwetware related====
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*[[OpenWetWare:Welcome|Introductory tutorial]]
 
*[[OpenWetWare:Welcome|Introductory tutorial]]
 
*[[Help|OpenWetWare help pages]]
 
*[[Help|OpenWetWare help pages]]
*[[Notebook|My Notebook]]
 
 
 
  
 +
====Technical guides====
  
*[[Lauber: I won't forget|I won't forget a thing]]
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*[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide]

Revision as of 08:58, 6 November 2012

Contact Info

  • sean.laub@gmail.com

Education

  • M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
  • B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
  • DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004

Protocols

Richards Lab Protocols

Molecular Techniques
Mammalian Cell Culture
Technical
Animal Work

Joyce Lab Protocols

Links

Protocols and general information

Useful links

Openwetware related

Technical guides